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Journal Article

RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis

MPS-Authors
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Christie,  M
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Karaaslan,  ES
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Lang,  PLM
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Weigel,  D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Lange, H., Ndecky, S., Gomez-Diaz, C., Pflieger, D., Butel, N., Zumsteg, J., et al. (2019). RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis. Nature Communications, 10: 3871. doi:10.1038/s41467-019-11807-4.


Cite as: https://hdl.handle.net/21.11116/0000-000A-6685-D
Abstract
The RNA exosome is a key 3'-5' exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.