A novel Ca 2+-binding protein influences photosynthetic electron transport in 
Anabaena sp. PCC 7120

Walter, J; Selim, KA; Leganés, F; Fernández-Piñas, F; Vothknecht, UC; Forchhammer, K; Aro, E-M; Gollan, PJ

doi:10.1016/j.bbabio.2019.04.007

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A novel Ca 2+-binding protein influences photosynthetic electron transport in Anabaena sp. PCC 7120

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Selim, KA
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Walter, J., Selim, K., Leganés, F., Fernández-Piñas, F., Vothknecht, U., Forchhammer, K., et al. (2019). A novel Ca 2+-binding protein influences photosynthetic electron transport in Anabaena sp. PCC 7120. Biochimica et Biophysica Acta, Bioenergetics, 1860(6), 519-532. doi:10.1016/j.bbabio.2019.04.007.

Cite as: https://hdl.handle.net/21.11116/0000-000A-68EF-5

Abstract

Ca2+ is a potent signalling molecule that regulates many cellular processes. In cyanobacteria, Ca2+ has been linked to cell growth, stress response and photosynthesis, and to the development of specialist heterocyst cells in certain nitrogen-fixing species. Despite this, the pathways of Ca2+ signal transduction in cyanobacteria are poorly understood, and very few protein components are known. The current study describes a previously unreported Ca2+-binding protein which was called the Ca2+ Sensor EF-hand (CSE), which is conserved in filamentous, nitrogen-fixing cyanobacteria. CSE is shown to bind Ca2+, which induces a conformational change in the protein structure. Poor growth of a strain of Anabaena sp. PCC 7120 overexpressing CSE was attributed to diminished photosynthetic performance. Transcriptomics, biophysics and proteomics analyses revealed modifications in the light-harvesting phycobilisome and photosynthetic reaction centre protein complexes.