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Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data

MPG-Autoren
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Bağci,  C
IMPRS From Molecules to Organisms, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271373

Buchfink,  B
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Zitation

Arumugam, K., Bağci, C., Bessarab, I., Beier, S., Buchfink, B., Górska, A., et al. (2019). Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data. Microbiome, 7: 61. doi:10.1186/s40168-019-0665-y.


Zitierlink: https://hdl.handle.net/21.11116/0000-000A-68FA-8
Zusammenfassung
ackground: Short-read sequencing technologies have long been the work-horse of microbiome analysis. Continuing technological advances are making the application of long-read sequencing to metagenomic samples increasingly feasible.
Results: We demonstrate that whole bacterial chromosomes can be obtained from an enriched community, by application of MinION sequencing to a sample from an EBPR bioreactor, producing 6 Gb of sequence that assembles into multiple closed bacterial chromosomes. We provide a simple pipeline for processing such data, which includes a new approach to correcting erroneous frame-shifts.
Conclusions: Advances in long-read sequencing technology and corresponding algorithms will allow the routine extraction of whole chromosomes from environmental samples, providing a more detailed picture of individual members of a microbiome.