Automated Detection of Autophagy Response Using Single Cell-Based Microscopy 
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Mueller, AJ; Proikas-Cezanne, T

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Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays

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Mueller, AJ
IMPRS From Molecules to Organisms, Max Planck Institute for Developmental Biology, Max Planck Society;

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Proikas-Cezanne, T
IMPRS From Molecules to Organisms, Max Planck Institute for Developmental Biology, Max Planck Society;

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Mueller, A., & Proikas-Cezanne, T. (2019). Automated Detection of Autophagy Response Using Single Cell-Based Microscopy Assays. In Methods in Molecular Biology. US: Springer.

Cite as: https://hdl.handle.net/21.11116/0000-000A-6A0B-4

Abstract

The fluorescence microscopy-based detection of intracellular LC3, p62, and/or WIPI punctate structures is a robust tool to monitor and assess macroautophagy/autophagy in single cells. This method was established for automated high-throughput/content analysis to reliably detect narrow differences in autophagy activity/capacity and to provide screening opportunities for biological and chemical libraries.