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Book Chapter

Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR

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Kaster,  M
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Laubinger,  S
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Kaster, M., & Laubinger, S. (2016). Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR. In Methods in Molecular Biology. US: Springer.


Cite as: https://hdl.handle.net/21.11116/0000-000A-80A6-9
Abstract
Nucleosome occupancy in promoter and genic regions can severely influence the transcription levels. Few methods have been established to investigate the nucleosome occupancy along the DNA. In this chapter we describe a detailed protocol to analyze the nucleosome occupancy at a specific locus using MNase-pPCR.