Live fluorescence imaging of F-actin organization in chick whole embryo 
cultures using SiR-actin

Schmitz-Elbers, M.; Lukinavicius, G.; Smit, T. H.

doi:10.3390/cells10071578

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Live fluorescence imaging of F-actin organization in chick whole embryo cultures using SiR-actin

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Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Citation

Schmitz-Elbers, M., Lukinavicius, G., & Smit, T. H. (2021). Live fluorescence imaging of F-actin organization in chick whole embryo cultures using SiR-actin. Cells, 10: 1578. doi:10.3390/cells10071578.

Cite as: https://hdl.handle.net/21.11116/0000-000A-8D7C-D

Abstract

Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.