English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Genomic integration of transgenes using UV irradiation in Pristionchus pacificus

MPS-Authors
/persons/resource/persons272221

Han,  Z
Department Integrative Evolutionary Biology, Max Planck Institute for Biology Tübingen, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Eren, G., Roca, M., Han, Z., & Lightfoot, J. (2022). Genomic integration of transgenes using UV irradiation in Pristionchus pacificus. microPublication Biology, 2022: 000576. doi:10.17912/micropub.biology.000576.


Cite as: https://hdl.handle.net/21.11116/0000-000A-9649-B
Abstract
Transgenes are widely used throughout molecular biology for numerous applications. In Caenorhabditis elegans, stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays. Furthermore, numerous technologies exist to integrate transgenes into the C. elegans genome. In the nematode Pristionchus pacificus, transgenes are possible, however, their establishment is less efficient and dependent on the formation of complex arrays containing the transgene of interest and host carrier DNA. Additionally, genomic integration has only been reported via biolistic methods. Here we describe a simple technique using UV irradiation to facilitate the integration of transgenes into the P. pacificus genome.