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Confocal Interferometric Scattering Microscopy Reveals 3D Nanoscopic Structure and Dynamics in Live Cells

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/persons/resource/persons270623

Küppers,  Michelle
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Sandoghdar Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Department of Physics, Friedrich-Alexander-Universit ̈at Erlangen-Nürnberg;

/persons/resource/persons276810

Albrecht,  David
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Sandoghdar Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons270611

Kashkanova,  Anna D.
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Sandoghdar Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons277810

Lühr,  Jennifer
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Sandoghdar Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons201175

Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Sandoghdar Division, Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;
Department of Physics, Friedrich-Alexander-Universit ̈at Erlangen-Nürnberg;

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s41467-023-37497-7.pdf
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Citation

Küppers, M., Albrecht, D., Kashkanova, A. D., Lühr, J., & Sandoghdar, V. (2023). Confocal Interferometric Scattering Microscopy Reveals 3D Nanoscopic Structure and Dynamics in Live Cells. Nature Communications, 14: 1962 (2023). doi:10.1038/s41467-023-37497-7.


Cite as: https://hdl.handle.net/21.11116/0000-000A-A251-3
Abstract
Bright-field light microscopy and related techniques continue to play a key role in life sciences because they provide a facile and label-free insight into biological specimen. However, lack of three-dimensional imaging and low sensitivity to nanoscopic features hamper their application in high-end quantitative studies. Here, we remedy these shortcomings by employing confocal interferometric scattering (iSCAT) microscopy. We demonstrate the performance of this label-free technique in a selection of case studies in live cells and benchmark our findings against simultaneously acquired fluorescence images. We reveal the nanometric topography of the nuclear envelope, quantify the dynamics of the endoplasmic reticulum, detect single microtubules, and map nanoscopic diffusion of clathrin-coated pits undergoing endocytosis. Furthermore, we introduce the combination of confocal and wide-field iSCAT modalities for simultaneous imaging of cellular structures and high-speed tracking of nanoscopic entities such as single SARS-CoV2 virions. Confocal iSCAT can be readily implemented as an additional contrast mechanism in existing laser scanning microscopes.