Towards a molecular understanding of microRNA-mediated gene silencing

Jonas, S; Izaurralde, E

doi:10.1038/nrg3965

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Towards a molecular understanding of microRNA-mediated gene silencing

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Jonas, S
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Izaurralde, E
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Jonas, S., & Izaurralde, E. (2015). Towards a molecular understanding of microRNA-mediated gene silencing. Nature Reviews Genetics, 16(7), 421-433. doi:10.1038/nrg3965.

Cite as: https://hdl.handle.net/21.11116/0000-000A-A359-A

Abstract

MicroRNAs (miRNAs) are a conserved class of small non-coding RNAs that assemble with Argonaute proteins into miRNA-induced silencing complexes (miRISCs) to direct post-transcriptional silencing of complementary mRNA targets. Silencing is accomplished through a combination of translational repression and mRNA destabilization, with the latter contributing to most of the steady-state repression in animal cell cultures. Degradation of the mRNA target is initiated by deadenylation, which is followed by decapping and 5'-to-3' exonucleolytic decay. Recent work has enhanced our understanding of the mechanisms of silencing, making it possible to describe in molecular terms a continuum of direct interactions from miRNA target recognition to mRNA deadenylation, decapping and 5'-to-3' degradation. Furthermore, an intricate interplay between translational repression and mRNA degradation is emerging.