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Journal Article

Matrix-trapped viruses can prevent invasion of bacterial biofilms by colonizing cells

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Vidakovic,  Lucia
Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Singh,  Praveen K.
Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Drescher,  Knut
Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Biozentrum, University of Basel, Basel, Switzerland;
Department of Physics, Philipps University Marburg, Marburg, Germany;

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Citation

Bond, M. C., Vidakovic, L., Singh, P. K., Drescher, K., & Nadell, C. D. (2021). Matrix-trapped viruses can prevent invasion of bacterial biofilms by colonizing cells. eLife, 10: e65355. doi:10.7554/eLife.65355.


Cite as: https://hdl.handle.net/21.11116/0000-000A-A460-0
Abstract
Bacteriophages can be trapped in the matrix of bacterial biofilms, such that the cells inside them are protected. It is not known whether these phages are still infectious and whether they pose a threat to newly arriving bacteria. Here, we address these questions using Escherichia coli and its lytic phage T7. Prior work has demonstrated that T7 phages are bound in the outermost curli polymer layers of the E. coli biofilm matrix. We show that these phages do remain viable and can kill colonizing cells that are T7-susceptible. If cells colonize a resident biofilm before phages do, we find that they can still be killed by phage exposure if it occurs soon thereafter. However, if colonizing cells are present on the biofilm long enough before phage exposure, they gain phage protection via envelopment within curli-producing clusters of the resident biofilm cells.