Your personalized protein structure: Andrei N. Lupas fused to GCN4 adaptors

Deiss, S; Hernandez Alvarez, B; Bär, K; Ewers, CP; Coles, M; Albrecht, R; Hartmann, MD

doi:10.1016/j.jsb.2014.01.013

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Your personalized protein structure: Andrei N. Lupas fused to GCN4 adaptors

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Deiss, S
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;
Conservation of Protein Structure and Function Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Hernandez Alvarez, B
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;
Conservation of Protein Structure and Function Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Bär, K
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Ewers, CP
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Coles, M
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;
Transmembrane Signal Transduction Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Albrecht, R
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;
Molecular Recognition and Catalysis Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Hartmann, MD
Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;
Molecular Recognition and Catalysis Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Deiss, S., Hernandez Alvarez, B., Bär, K., Ewers, C., Coles, M., Albrecht, R., et al. (2014). Your personalized protein structure: Andrei N. Lupas fused to GCN4 adaptors. Journal of Structural Biology, 186(3), 380-385. doi:10.1016/j.jsb.2014.01.013.

Cite as: https://hdl.handle.net/21.11116/0000-000A-ABFB-B

Abstract

This work presents a protein structure that has been designed purely for aesthetic reasons, symbolizing decades of coiled-coil research and praising its most fundamental model system, the GCN4 leucine zipper. The GCN4 leucine zipper is a highly stable coiled coil which can be tuned to adopt different oligomeric states via mutation of its core residues. For these reasons it is used in structural studies as a stabilizing fusion adaptor. On the occasion of the 50th birthday of Andrei N. Lupas, we used it to create the first personalized protein structure: we fused the sequence ANDREI-N-LVPAS in heptad register to trimeric GCN4 adaptors and determined its structure by X-ray crystallography. The structure demonstrates the robustness and versatility of GCN4 as a fusion adaptor. We learn how proline can be accommodated in trimeric coiled coils, and put the structure into the context of the other GCN4-fusion structures known to date.