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Journal Article

LEGO-NMR spectroscopy: a method to visualize individual subunits in large heteromeric complexes

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Mund,  M
Research Group NMR Spectroscopy of Large Complexes, Max Planck Institute for Developmental Biology, Max Planck Society;

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Overbeck,  JH
Research Group NMR Spectroscopy of Large Complexes, Max Planck Institute for Developmental Biology, Max Planck Society;

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Ullmann,  J
Research Group NMR Spectroscopy of Large Complexes, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272110

Sprangers,  R
Research Group NMR Spectroscopy of Large Complexes, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Mund, M., Overbeck, J., Ullmann, J., & Sprangers, R. (2013). LEGO-NMR spectroscopy: a method to visualize individual subunits in large heteromeric complexes. Angewandte Chemie, International Edition in English, 52(43), 11401-11405. doi:10.1002/anie.201304914.


Cite as: https://hdl.handle.net/21.11116/0000-000A-ACC6-5
Abstract
Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in only a subset of their subunits can be prepared, thus decreasing NMR spectral complexity. For the hetero heptameric LSm1-7 and LSm2-8 rings NMR spectra of the individual subunits of the complete complex are obtained, showing a conserved RNA binding site. This LEGO-NMR technique makes large asymmetric complexes accessible to detailed NMR spectroscopic studies.