English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

chokh/rx3 specifies the retinal pigment epithelium fate independently of eye morphogenesis

MPS-Authors
/persons/resource/persons275872

Rojas-Muñoz,  A
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons275870

Dahm,  R
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard,  C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Rojas-Muñoz, A., Dahm, R., & Nüsslein-Volhard, C. (2005). chokh/rx3 specifies the retinal pigment epithelium fate independently of eye morphogenesis. Developmental Biology, 288(2), 348-326. doi:10.1016/j.ydbio.2005.08.046.


Cite as: http://hdl.handle.net/21.11116/0000-000A-B094-7
Abstract
Despite the importance of the retinal pigment epithelium (RPE) for vision, the molecular processes involved in its specification are poorly understood. We identified two new mutant alleles for the zebrafish gene chokh (chk), which display a reduction or absence of the RPE. Unexpectedly, the neural retina (NR) in chk is specified and laminated, indicating that the regulatory network leading to NR development is largely independent of the RPE. Genetic mapping and molecular characterization revealed that chk encodes Rx3. Expression analyses show that otx2 and mitfb are not expressed in the prospective RPE of chk, indicating that the retinal homeobox gene rx3 acts upstream of the molecular network controlling RPE specification. Cellular transplantations demonstrate that rx3 function is autonomously required to specify the prospective RPE. Though rx2 is also absent in chk, neither rx2 nor rx1 is required for RPE development. Thus, our data provide the first indication that, in addition to controlling optic lobe evagination and proliferation, chk/rx3 also determines cellular fate.