1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 
1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum

Warlick, B. P.; Evans, B. S.; Erb, T. J.; Ramagopal, U. A.; Sriram, J.; Imker, H. J.; Sauder, J. M.; Bonanno, J. B.; Burley, S. K.; Tabita, F. R.; Almo, S. C.; Sweedler, J. S.; Gerlt, J. A.

doi:10.1021/bi301215g

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

https://www.ncbi.nlm.nih.gov/pubmed/23035785
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Warlick, B. P., Evans, B. S., Erb, T. J., Ramagopal, U. A., Sriram, J., Imker, H. J., et al. (2012). 1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum. Biochemistry, 51(42), 8324-6. doi:10.1021/bi301215g.

Cite as: https://hdl.handle.net/21.11116/0000-000A-CB39-2

Abstract

Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.