English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells

MPS-Authors
/persons/resource/persons252968

Di Vittori,  V.
Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97147

Fernie,  A. R.
Central Metabolism, Department Gutjahr, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Otero, S., Gildea, I., Roszak, P., Lu, Y., Di Vittori, V., Bourdon, M., et al. (2022). A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells. Nature Plants, 8, 954-970. doi:10.1038/s41477-022-01178-y.


Cite as: https://hdl.handle.net/21.11116/0000-000A-D2CE-1
Abstract
Single-cell sequencing has recently allowed the generation of exhaustive root cell atlases. However, some cell types are elusive and remain underrepresented. Here we use a second-generation single-cell approach, where we zoom in on the root transcriptome sorting with specific markers to profile the phloem poles at an unprecedented resolution. Our data highlight the similarities among the developmental trajectories and gene regulatory networks common to protophloem sieve element (PSE)-adjacent lineages in relation to PSE enucleation, a key event in phloem biology. As a signature for early PSE-adjacent lineages, we have identified a set of DNA-binding with one finger (DOF) transcription factors, the PINEAPPLEs (PAPL), that act downstream of PHLOEM EARLY DOF (PEAR) genes and are important to guarantee a proper root nutrition in the transition to autotrophy. Our data provide a holistic view of the phloem poles that act as a functional unit in root development.