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Mimicry technology: suppressing small RNA activity in plants

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Rubio-Somoza,  I
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Manavella,  PA
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Rubio-Somoza, I., & Manavella, P. (2011). Mimicry technology: suppressing small RNA activity in plants. In Methods in Molecular Biology. US: Springer.


Cite as: https://hdl.handle.net/21.11116/0000-000A-D8ED-8
Abstract
Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the above-mentioned limitation (Nat Genet 39:1033-1037, 2007). We engineered the IPS1 non-coding RNA to bear a complementary sequence to a given miRNA family, resulting in specific sequestration of RISC complexes. MIMIC technology allows for the constitutive release of all of the potential targets of a miRNA family as well as tissue-specific and inducible suppression of its activity.