Electrochemical Antigenic Sensor for the Diagnosis of Chronic Q Fever

Mathioudaki, Eirini; Alifragis, Yiannis; Fouskaki, Maria; Chochlakis, Dimosthenis; Xie, Hao; Psaroulaki, Anna; Tsiotis, Georgios; Chaniotakis, Nikolaos

doi:10.1016/j.crbiot.2022.10.006

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Electrochemical Antigenic Sensor for the Diagnosis of Chronic Q Fever

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Xie, Hao
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Mathioudaki, E., Alifragis, Y., Fouskaki, M., Chochlakis, D., Xie, H., Psaroulaki, A., et al. (2022). Electrochemical Antigenic Sensor for the Diagnosis of Chronic Q Fever. Current Research in Biotechnology, 4, 537-543. doi:10.1016/j.crbiot.2022.10.006.

Cite as: https://hdl.handle.net/21.11116/0000-000A-E146-9

Abstract

In this work, we report the development of an impedimetric biosensor for the direct, quick, and easy diagnosis of chronic Q fever. The biosensor is based on highly sensitive antigens that can selectively recognize antibodies against Coxiella burnetii. The biosensor is based on the immobilization of antigens onto a gold electrode using the EDC/NHS immobilization methodology. The detection is performed by impedance spectroscopy that monitors specific frequencies which provide the maximum sensitivity for the biosensor. Q fever antibodies that are present in the sera of patients interact selectively with the biosensor antigens, thereby altering the impedance of the biosensor surfaceand generating a large impedance change within a few seconds. The biosensor allows for the specific serological detection of chronic Q fever, while the developed system can also be modified for the detection of other biomarkers, such as the ones against acute Q fever.