Molecular cloning of a dominant roller mutant and establishment of DNA-mediated 
transformation in the nematode Pristionchus pacificus

Schlager, B; Wang, X; Braach, G; Sommer, RJ

doi:10.1002/dvg.20499

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Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus

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Schlager, B
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Wang, X
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Braach, G
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Sommer, RJ
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Schlager, B., Wang, X., Braach, G., & Sommer, R. (2009). Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus. Genesis, 47(5), 300-304. doi:10.1002/dvg.20499.

Cite as: https://hdl.handle.net/21.11116/0000-000A-EE25-1

Abstract

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus.