Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell 
imaging.

Gabriele, Michele; Brandão, Hugo B; Grosse-Holz, Simon; Jha, Asmita; Dailey, Gina M; Cattoglio, Claudia; Hsieh, Tsung-Han S; Mirny, Leonid; Zechner, Christoph; Hansen, Anders S

doi:10.1126/science.abn6583

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Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging.

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Zechner, Christoph
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Gabriele, M., Brandão, H. B., Grosse-Holz, S., Jha, A., Dailey, G. M., Cattoglio, C., et al. (2022). Dynamics of CTCF- and cohesin-mediated chromatin looping revealed by live-cell imaging. Science (New York, N.Y.), 376(6592), 496-501. doi:10.1126/science.abn6583.

Cite as: https://hdl.handle.net/21.11116/0000-000B-0335-6

Abstract

Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and loop-extruding cohesins, but the live dynamics of loop formation and stability remain unknown. Here, we directly visualized chromatin looping at the Fbn2 TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantified looping dynamics by Bayesian inference. Unexpectedly, the Fbn2 loop was both rare and dynamic, with a looped fraction of approximately 3 to 6.5% and a median loop lifetime of approximately 10 to 30 minutes. Our results establish that the Fbn2 TAD is highly dynamic, and about 92% of the time, cohesin-extruded loops exist within the TAD without bridging both CTCF boundaries. This suggests that single CTCF boundaries, rather than the fully CTCF-CTCF looped state, may be the primary regulators of functional interactions.