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Abstract

The non-random three-dimensional organization of genomes is critical for many cellular processes. Recently, analyses of genome-wide
chromatin packing in the model dicot plant Arabidopsis thaliana have been reported. At a kilobase scale, the A. thaliana chromatin interaction
network is highly correlated with a range of genomic and epigenomic features. Surprisingly, Topologically Associated Domains (TADs), which
appear to be a prevalent structural feature of genome packing in many animal species, are not prominent in the A. thaliana genome. Using a
genome-wide chromatin conformation capture approach, Hi-C, we have determined high-resolution chromatin packing patterns of rice. We
found new structural features of chromatin organization at both chromosomal and local levels compared to A. thaliana, with thousands of
distinct TADs that cover about a quarter of the rice genome. The rice TAD boundaries are associated with euchromatic epigenetic marks and
active gene expression, and enriched with a sequence motif that can be recognized by plant-specific TCP proteins. In addition, we report
chromosome decondensation in rice seedlings undergoing cold stress, despite local chromatin packing patterns remaining largely unchanged.
The substantial variation found already in a comparison of two plant species suggests that chromatin organization in plants might be more diverse than in multicellular animals.