Isolation of mutations with dumpy-like phenotypes and of collagen genes in the 
nematode Pristionchus pacificus

Kenning, C; Kipping, I; Sommer, RJ

doi:10.1002/gene.20084

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Isolation of mutations with dumpy-like phenotypes and of collagen genes in the nematode Pristionchus pacificus

MPS-Authors

/persons/resource/persons281445

Kenning, C
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons281447

Kipping, I
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271084

Sommer, RJ
Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Kenning, C., Kipping, I., & Sommer, R. (2004). Isolation of mutations with dumpy-like phenotypes and of collagen genes in the nematode Pristionchus pacificus. Genesis, 40(3), 176-183. doi:10.1002/gene.20084.

引用: https://hdl.handle.net/21.11116/0000-000B-39FE-8

要旨

The nematode Pristionchus pacificus was developed as a satellite system in evolutionary developmental biology and forward and reverse genetic approaches allow a detailed comparison of various developmental processes between P. pacificus and Caenorhabditis elegans. To facilitate map-based cloning in P. pacificus, a genome map was generated including a genetic linkage map of approximately 300 molecular markers and a physical map of 10,000 BAC clones. Here, we describe the isolation and characterization of more than 40 morphological mutations that can be used as genetic markers. These mutations fall into 12 Dumpy genes and one Roller gene that represent morphological markers for all six P. pacificus chromosomes. Using an in silico approach, we identified approximately 150 hits of P. pacificus collagen genes in the available EST, BAC-end, and fosmid-end sequences. However, 1:1 orthologs could only be identified for fewer than 20 collagen genes.