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Abstract

Primary cultures of hippocampal neurons were infected with an adenovirus coding for P-galactosidase. Expression could be detected as early as 4 h after infection and steadily increased to high levels at 24 h without evidence for a functional impairment of the infected neurons. Similarly, adenovirus-mediated gene transfer into acute hippocampal slices was detectable 4 h after infection and could be localized to discrete areas of the CAI region by microinjection of the virus stock solution. Infected slices were still suitable for electrophysiological experiments.