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Cover Feature: Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement (ChemPhysChem 19/2022)

MPS-Authors
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Shoeman,  Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Barends,  Thomas R. M.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Bezchastnov,  Victor
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Doak,  R. Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Foucar,  Lutz
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Gorel,  Alexander
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Grünbein,  Marie Luise
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Hilpert,  Mario
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Kloos,  Marco
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Nass,  Karol
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Nass Kovacs,  Gabriela
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Roome,  Christopher M.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Schlichting,  Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Adam, V., Hadjidemetriou, K., Jensen, N., Shoeman, R. L., Woodhouse, J., Aquila, A., et al. (2022). Cover Feature: Rational control of structural off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement (ChemPhysChem 19/2022). ChemPhysChem, 23(19): e202200693, pp. 1-1. doi:10.1002/cphc.202200693.


Cite as: https://hdl.handle.net/21.11116/0000-000B-4275-7
Abstract
The Cover Feature displays a comparison of chromophore conformations in the X-ray crystal structures of the reversibly photoswitchable fluorescent protein rsEGFP2 in its on state (green) and its V151L (purple) and V151A (yellow) variants in their off states. The latter displays an increased photoswitching contrast, as indicated by the displayed switching kinetics. Cover design by Virgile ADAM. More information can be found in the Research Article by Dominique Bourgeois, Martin Weik and co-workers.