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Barcoding populations of Pseudomonas fluorescens SBW25

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Theodosiou,  Loukas
Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Farr,  Andrew
Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Rainey,  Paul B.       
Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Theodosiou, L., Farr, A., & Rainey, P. B. (2022). Barcoding populations of Pseudomonas fluorescens SBW25. Journal of Molecular Evolution: the Journal of the International Society of Molecular Evolution, 91, 254-262. doi:10.1007/s00239-023-10103-6.


Cite as: https://hdl.handle.net/21.11116/0000-000B-43F0-A
Abstract
In recent years evolutionary biologists have developed increasing interest in the use of barcoding strategies to study eco-evolutionary dynamics of lineages within evolving populations and communities. Although barcoded populations can deliver unprecedented insight into evolutionary change, barcoding microbes presents specific technical challenges. Here, strategies are described for barcoding populations of the model bacterium Pseudomonas fluorescens SBW25, including the design and cloning of barcoded regions, preparation of libraries for amplicon sequencing, and quantification of resulting barcoded lineages. In so doing, we hope to aid the design and implementation of barcoding methodologies in a broad range of model and non-model organisms.Competing Interest StatementThe authors have declared no competing interest.