Transformation of agrobacterium using the freeze-thaw method

Weigel, D; Glazebrook, J

doi:10.1101/pdb.prot4666

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Transformation of agrobacterium using the freeze-thaw method

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Weigel, D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Weigel, D., & Glazebrook, J. (2006). Transformation of agrobacterium using the freeze-thaw method. Cold Spring Harbor protocols, 2006(7): pdb.prot4666. doi:10.1101/pdb.prot4666.

Cite as: https://hdl.handle.net/21.11116/0000-000B-44F6-3

Abstract

This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.