Transformation of agrobacterium using electroporation

Weigel, D; Glazebrook, J

doi:10.1101/pdb.prot4665

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Transformation of agrobacterium using electroporation

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Weigel, D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Weigel, D., & Glazebrook, J. (2006). Transformation of agrobacterium using electroporation. Cold Spring Harbor protocols, 2006(7): pdb.prot4665. doi:10.1101/pdb.prot4665.

Cite as: https://hdl.handle.net/21.11116/0000-000B-44F8-1

Abstract

This protocol describes a method for transforming Agrobacterium with plasmid DNA using electroporation in a manner similar to that commonly used for Escherichia coli. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of Agrobacterium transformants with this technique.