Kanamycin selection of transformed Arabidopsis

Weigel, D; Glazebrook, J

doi:10.1101/pdb.prot4669

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Kanamycin selection of transformed Arabidopsis

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Weigel, D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Weigel, D., & Glazebrook, J. (2006). Kanamycin selection of transformed Arabidopsis. Cold Spring Harbor protocols, 2006(7): pdb.prot4669. doi:10.1101/pdb.prot4669.

Cite as: https://hdl.handle.net/21.11116/0000-000B-4502-5

Abstract

The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this protocol, kanamycin-resistant seedlings are selected on solid medium.