Vectors and agrobacterium hosts for Arabidopsis transformation

Weigel, D; Glazebrook, J

doi:10.1101/pdb.ip29

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Vectors and agrobacterium hosts for Arabidopsis transformation

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Weigel, D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Weigel, D., & Glazebrook, J. (2006). Vectors and agrobacterium hosts for Arabidopsis transformation. Cold Spring Harbor protocols, 2006(7): pdb.ip29. doi:10.1101/pdb.ip29.

Cite as: https://hdl.handle.net/21.11116/0000-000B-4518-D

Abstract

Arabidopsis can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The T-DNA, which is flanked by left- and right-border (LB and RB) sequences, resides on a tumor-inducing (Ti) plasmid. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts.