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Journal Article

Glucogallin Attenuates the LPS-Induced Signaling in Macrophages and Protects Mice against Sepsis


Gautam,  A       
IMPRS From Molecules to Organisms, Max Planck Institute for Biology Tübingen, Max Planck Society;

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Singh, R., Chandel, S., Ghosh, A., Matta, T., Gautam, A., Bhattacharya, A., et al. (2022). Glucogallin Attenuates the LPS-Induced Signaling in Macrophages and Protects Mice against Sepsis. International Journal of Molecular Sciences, 23(19): 11254. doi:10.3390/ijms231911254.

Cite as: https://hdl.handle.net/21.11116/0000-000B-5952-5
The anti-oxidant and anti-inflammatory effect of beta-glucogallin (BGG), a plant-derived natural product, was evaluated in both in vitro and in vivo studies. For the in vitro study, the ability of BGG pre-treatment to quench LPS-induced effects compared to LPS alone in macrophages was investigated. It was found that BGG pre-treatment showed a significant decrease in ROS, NO, superoxide, and pro-inflammatory cytokines (TNF-alpha, IL-4, IL-17, IL-1β, and IL-6) and increased reduced glutathione coupled with the restoration of mitochondrial membrane potential. Gene profiling and further validation by qPCR showed that BGG pre-treatment downregulated the LPS-induced expression of c-Fos, Fas, MMP-9, iNOS, COX-2, MyD88, TRIF, TRAF6, TRAM, c-JUN, and NF-κB. We observed that BGG pre-treatment reduced nuclear translocation of LPS-activated NF-κB and thus reduced the subsequent expressions of NLRP3 and IL-1β, indicating the ability of BGG to inhibit inflammasome formation. Molecular docking studies showed that BGG could bind at the active site of TLR4. Finally, in the LPS-driven sepsis mouse model, we showed that pre-treatment with BGG sustained toxic shock, as evident from their 100% survival. Our study clearly showed the therapeutic potential of BGG in toxic shock syndrome.