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Journal Article

Purification and identification of endogenous polySUMO conjugates


Matić,  I.
Matic – ADP-ribosylation in DNA Repair and Ageing, Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society;

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Bruderer, R., Tatham, M. H., Plechanovova, A., Matić, I., Garg, A. K., & Hay, R. T. (2011). Purification and identification of endogenous polySUMO conjugates. EMBO Rep, 12(2), 142-8. doi:10.1038/embor.2010.206.

Cite as: https://hdl.handle.net/21.11116/0000-000B-A96C-E
The small ubiquitin-like modifier (SUMO) can undergo self-modification to form polymeric chains that have been implicated in cellular processes such as meiosis, genome maintenance and stress response. Investigations into the biological role of polymeric chains have been hampered by the absence of a protocol for the purification of proteins linked to SUMO chains. In this paper, we describe a rapid affinity purification procedure for the isolation of endogenous polySUMO-modified species that generates highly purified material suitable for individual protein studies and proteomic analysis. We use this approach to identify more than 300 putative polySUMO conjugates from cultured eukaryotic cells.