Analysis of intron sequences reveals hallmarks of circular RNA biogenesis in 
animals

Ivanov, A.; Memczak, S.; Wyler, E.; Torti, F.; Porath, H. T.; Orejuela, M. R.; Piechotta, M.; Levanon, E. Y.; Landthaler, M.; Dieterich, C.; Rajewsky, N.

doi:10.1016/j.celrep.2014.12.019

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Analysis of intron sequences reveals hallmarks of circular RNA biogenesis in animals

MPS-Authors

/persons/resource/persons129352

Dieterich, C.
Dieterich – Computational RNA Biology and Ageing, Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society;

External Resource

http://www.ncbi.nlm.nih.gov/pubmed/25558066
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Ivanov, A., Memczak, S., Wyler, E., Torti, F., Porath, H. T., Orejuela, M. R., et al. (2015). Analysis of intron sequences reveals hallmarks of circular RNA biogenesis in animals. Cell Rep, 10(2), 170-7. doi:10.1016/j.celrep.2014.12.019.

Cite as: https://hdl.handle.net/21.11116/0000-000B-83EE-5

Abstract

Circular RNAs (circRNAs) are a large class of animal RNAs. To investigate possible circRNA functions, it is important to understand circRNA biogenesis. Besides human ALU repeats, sequence features that promote exon circularization are largely unknown. We experimentally identified circRNAs in C. elegans. Reverse complementary sequences between introns bracketing circRNAs were significantly enriched in comparison to linear controls. By scoring the presence of reverse complementary sequences in human introns, we predicted and experimentally validated circRNAs. We show that introns bracketing circRNAs are highly enriched in RNA editing or hyperediting events. Knockdown of the double-strand RNA-editing enzyme ADAR1 significantly and specifically upregulated circRNA expression. Together, our data support a model of animal circRNA biogenesis in which competing RNA-RNA interactions of introns form larger structures that promote circularization of embedded exons, whereas ADAR1 antagonizes circRNA expression by melting stems within these interactions.