The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion 
dynamics during cell migration

Schiefermeier, Natalia; Scheffler, Julia M.; de Araujo, M. E. G.; Stasyk, T.; Yordanov, T.; Ebner, H. L.; Offterdinger, M.; Munck, S.; Hess, M. W.; Wickström, S. A.; Lange, A.; Wunderlich, W.; Faessler, R.; Teis, D.; Huber, L. A.

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration

MPS-Authors

/persons/resource/persons129350

Wickström, S. A.
Wickström – Skin Homeostasis and Ageing, Max Planck Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society;

External Resource

http://www.ncbi.nlm.nih.gov/pubmed/24841562
(全文テキスト（全般）)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Schiefermeier, N., Scheffler, J. M., de Araujo, M. E. G., Stasyk, T., Yordanov, T., Ebner, H. L., Offterdinger, M., Munck, S., Hess, M. W., Wickström, S. A., Lange, A., Wunderlich, W., Faessler, R., Teis, D., & Huber, L. A. (2014). The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. JOURNAL OF CELL BIOLOGY, 205(4), 525-540.

引用: https://hdl.handle.net/21.11116/0000-000B-722C-4

要旨

Cell migration is mediated by the dynamic remodeling of focal adhesions (FAs). Recently, an important role of endosomal signaling in regulation of cell migration was recognized. Here, we show an essential function for late endosomes carrying the p14-MP1 (LAMTOR2/3) complex in FA dynamics. p14-MP1-positive endosomes move to the cell periphery along microtubules (MTs) in a kinesin1- and Arl8b-dependent manner. There they specifically target FAs to regulate FA turnover, which is required for cell migration. Using genetically modified fibroblasts from p14-deficient mice and Arl8b-depleted cells, we demonstrate that MT plus end-directed traffic of p14-MP1-positive endosomes triggered IQGAP1 disassociation from FAs. The release of IQGAP was required for FA dynamics. Taken together, our results suggest that late endosomes contribute to the regulation of cell migration by transporting the p14-MP1 scaffold complex to the vicinity of FAs.