Insight into hepatocellular carcinogenesis at transcriptome level by comparing 
gene expression profiles of hepatocellular carcinoma with those of 
corresponding noncancerous liver

Xu, X.; Huang, J.; Xu, Z. G.; Qian, B. Z.; Zhu, Z. D.; Yan, Q.; Cai, T.; Zhang, X.; Xiao, H. S.; Qu, J.; Liu, F.; Huang, Q. H.; Cheng, Z. H.; Li, N. G.; Du, J. J.; Hu, W.; Shen, K. T.; Lu, G.; Fu, G.; Zhong, M.; Xu, S. H.; Gu, W. Y.; Huang, W.; Zhao, X. T.; Hu, G. X.; Gu, J. R.; Chen, Z.; Han, Z. G.

doi:10.1073/pnas.241522398

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学術論文

Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver

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Xu, X.
Xu – Epigenetic Regulation of Mammalian Ageing, Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/11752456
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引用

Xu, X., Huang, J., Xu, Z. G., Qian, B. Z., Zhu, Z. D., Yan, Q., Cai, T., Zhang, X., Xiao, H. S., Qu, J., Liu, F., Huang, Q. H., Cheng, Z. H., Li, N. G., Du, J. J., Hu, W., Shen, K. T., Lu, G., Fu, G., Zhong, M., Xu, S. H., Gu, W. Y., Huang, W., Zhao, X. T., Hu, G. X., Gu, J. R., Chen, Z., & Han, Z. G. (2001). Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver. Proc Natl Acad Sci U S A, 98(26), 15089-94. doi:10.1073/pnas.241522398.

引用: https://hdl.handle.net/21.11116/0000-000B-68EB-8

要旨

Human hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. In this work, we report on a comprehensive characterization of gene expression profiles of hepatitis B virus-positive HCC through the generation of a large set of 5'-read expressed sequence tag (EST) clusters (11,065 in total) from HCC and noncancerous liver samples, which then were applied to a cDNA microarray system containing 12,393 genes/ESTs and to comparison with a public database. The commercial cDNA microarray, which contains 1,176 known genes related to oncogenesis, was used also for profiling gene expression. Integrated data from the above approaches identified 2,253 genes/ESTs as candidates with differential expression. A number of genes related to oncogenesis and hepatic function/differentiation were selected for further semiquantitative reverse transcriptase-PCR analysis in 29 paired HCC/noncancerous liver samples. Many genes involved in cell cycle regulation such as cyclins, cyclin-dependent kinases, and cell cycle negative regulators were deregulated in most patients with HCC. Aberrant expression of the Wnt-beta-catenin pathway and enzymes for DNA replication also could contribute to the pathogenesis of HCC. The alteration of transcription levels was noted in a large number of genes implicated in metabolism, whereas a profile change of others might represent a status of dedifferentiation of the malignant hepatocytes, both considered as potential markers of diagnostic value. Notably, the altered transcriptome profiles in HCC could be correlated to a number of chromosome regions with amplification or loss of heterozygosity, providing one of the underlying causes of the transcription anomaly of HCC.