Ten-eleven translocation (Tet) and thymine DNA glycosylase (TDG), components of 
the demethylation pathway, are direct targets of miRNA-29a

Zhang, P.; Huang, B.; Xu, X.; Sessa, W. C.

doi:10.1016/j.bbrc.2013.06.082

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Ten-eleven translocation (Tet) and thymine DNA glycosylase (TDG), components of the demethylation pathway, are direct targets of miRNA-29a

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Xu, X.
Xu – Epigenetic Regulation of Mammalian Ageing, Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/23820384
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Citation

Zhang, P., Huang, B., Xu, X., & Sessa, W. C. (2013). Ten-eleven translocation (Tet) and thymine DNA glycosylase (TDG), components of the demethylation pathway, are direct targets of miRNA-29a. Biochem Biophys Res Commun, 437(3), 368-73. doi:10.1016/j.bbrc.2013.06.082.

Cite as: https://hdl.handle.net/21.11116/0000-000B-68D1-4

Abstract

The ten-eleven translocation family of proteins (Tet1/2/3, Tets) converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which can be further oxidized and repaired by thymine DNA glycosylase (TDG), to influence gene transcription in embryonic and adult tissues. However the mechanisms of how Tets and TDG levels are regulated are unknown. We show that miR-29 can directly regulate Tet1-3 and TDG mRNA levels through binding to their 3'UTRs. miR-29 mimic decreases global 5hmC levels, a hallmark of Tet activity. Moreover, the mRNA levels for Tet3 and TDG are inversely correlated with the levels of miR-29 in aged mouse aorta implying that aging may affect methylation patterns via miRNA. In summary, our data show that Tets and TDG are direct targets of miR-29 and unravel a novel regulatory role for this miRNA in epigenetic DNA demethylation pathways.