Cytosolic Ca2+ Acts by Two Separate Pathways to Modulate the Supply of 
Release-Competent Vesicles in Chromaffin Cells

Smith, C.; Moser, T.; Xu, T.; Neher, Erwin

doi:10.1016/S0896-6273(00)80504-8

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Cytosolic Ca²⁺ Acts by Two Separate Pathways to Modulate the Supply of Release-Competent Vesicles in Chromaffin Cells

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Smith, C.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Moser, T.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

Xu, T.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Neher, Erwin
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Smith, C., Moser, T., Xu, T., & Neher, E. (1998). Cytosolic Ca²⁺ Acts by Two Separate Pathways to Modulate the Supply of Release-Competent Vesicles in Chromaffin Cells. Neuron, 20(6), 1243-1253. doi:10.1016/S0896-6273(00)80504-8.

Cite as: https://hdl.handle.net/21.11116/0000-000C-75AB-0

Abstract

Recovery from depletion of the readily releasable pool of vesicles (RRP) in adrenal chromaffin cells was studied at differing basal [Ca2+]i or following protein kinase C (PKC) activation by phorbol esters. Following depletion, the pool size was estimated at varied times from cell capacitance jumps in response to paired depolarizations. The experimentally observed RRP recovery time course and steady-state size could be predicted from the measured [Ca2+]i signal assuming a Michaelis-Menten-type regulation of the vesicle supply by Ca2+. An elevated recruitment activity was observed at increased [Ca2+]i even when protein kinase C was blocked, but maximum effects could be obtained only after stimulation of PKC by phorbol esters or by prolonged elevations in [Ca2+]i. We suggest that, in chromaffin cells, elevated cytosolic Ca2+ modulates exocytotic plasticity via PKC-dependent and -independent pathways.