English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Patch clamp techniques: An overview

MPS-Authors
/persons/resource/persons15570

Neher,  Erwin       
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Cahalan, M., & Neher, E. (1992). Patch clamp techniques: An overview. Methods in Enzymology, 207, 3-14. doi:10.1016/0076-6879(92)07003-7.


Cite as: https://hdl.handle.net/21.11116/0000-000C-8CDA-1
Abstract
This chapter provides an overview of the patch clamp techniques. The patch clamp technique provides the experimental means for merging the tools of modern molecular and cellular biology with those of electrophysiology. Using various recording configurations, it is possible to dissect the mechanisms of channel modulation. In cell-attached recording, modulation of channel activity in response to bath-applied agonist generally indicates a second-messenger mechanism. Candidate messengers can be tested directly on excised patches or in whole-cell recording. Current research on signaling pathways seeks to establish the functionally meaningful mechanisms through selective activation or inhibition of a portion of the pathway. A variety of patch clamp configurations, combined with single-channel resolution, provides a powerful experimental approach from the molecular level, in which channel genes are altered and expressed, to the cellular level, in which posttranslational signaling mechanisms are elucidated, to the systems level, in which cellular interactions in intact or slice preparations are revealed.