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Cover Feature: Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System (ChemBioChem 20/2019)

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Huertgen,  Daniel
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Mascarenhas,  Judita
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Murray,  Seán M.
Research Group Mechanisms of Spatial-Organisation, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Sourjik,  Victor
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Huertgen, D., Mascarenhas, J., Heymann, M., Murray, S. M., Schwille, P., & Sourjik, V. (2019). Cover Feature: Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System (ChemBioChem 20/2019).


Cite as: https://hdl.handle.net/21.11116/0000-000B-777E-3
Abstract
The replication and segregation of genetic material constitutes an essential feature for the design of a minimal synthetic cell. As depicted on the cover, we used T7 polymerase and the plasmid-derived ParMRC segregation system to establish these processes in vitro. These processes were incorporated into biomimetic compartments providing an enclosed reaction space. We further show that DNA replication and segregation could be coupled by using the condensed state of DNA nanoparticles, which emerge as a result of DNA replication. Overall, this work demonstrates an important step towards the development of a fully functional minimal cell. More information can be found in the full paper by D. Hürtgen, V. Sourjik, et al. on page 2633 in Issue 20, 2019 (DOI: 10.1002/cbic.201900299).