Replication of Phi-X174 DNA by Escherichia coli polA- in vitro (Phi-X174 
DNA-DNA replication-E. coli polA-)

Olivera, BM; Bonhoeffer, F

doi:10.1073/pnas.69.1.25

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Replication of Phi-X174 DNA by Escherichia coli polA- in vitro (Phi-X174 DNA-DNA replication-E. coli polA-)

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Bonhoeffer, F
Bonhoeffer Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Olivera, B., & Bonhoeffer, F. (1972). Replication of Phi-X174 DNA by Escherichia coli polA- in vitro (Phi-X174 DNA-DNA replication-E. coli polA-). Proceedings of the National Academy of Sciences of the United States of America, 69(1), 25-29. doi:10.1073/pnas.69.1.25.

Cite as: https://hdl.handle.net/21.11116/0000-000B-9D31-D

Abstract

Lysates of an Escherichia coli polA(-) strain convert single-stranded DNA from varphiX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.