Epithelial cell cluster size affects force distribution in response to 
EGF-induced collective contractility

Zambarda, Chiara; González, Carlos Pérez; Schoenit, Andreas; Veits, Nisha; Schimmer, Clara; Jung, Raimund; Ollech, Dirk; Christian, Joel; Roca-Cusachs, Pere; Trepat, Xavier; Cavalcanti-Adam, Elisabetta Ada

doi:10.1016/j.ejcb.2022.151274

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Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility

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Zambarda, Chiara
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons268418

Schoenit, Andreas
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons284227

Veits, Nisha
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons284229

Schimmer, Clara
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons284231

Jung, Raimund
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons125882

Ollech, Dirk
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons269240

Christian, Joel
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75354

Cavalcanti-Adam, Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Zambarda, C., González, C. P., Schoenit, A., Veits, N., Schimmer, C., Jung, R., et al. (2022). Epithelial cell cluster size affects force distribution in response to EGF-induced collective contractility. European Journal of Cell Biology: EJCB, 101(4): 151274, pp. 1-12. doi:10.1016/j.ejcb.2022.151274.

Cite as: https://hdl.handle.net/21.11116/0000-000B-B63A-7

Abstract

Several factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and directly activate the acto-myosin machinery. In this study we present the effects of EGF on the contractility of epithelial cancer cell colonies in confined geometry of different sizes. We show that the extent to which EGF triggers contractility scales with the cluster size and thus the number of cells. Moreover, the collective contractility results in a radial distribution of traction forces, which are dependent on integrin β1 peripheral adhesions and transmitted to neighboring cells through adherens junctions. Taken together, EGF-induced contractility acts on the mechanical crosstalk and linkage between the cell-cell and cell-matrix compartments, regulating collective responses.