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Journal Article

In vivo super-resolution of the brain - How to visualize the hidden nanoplasticity?


Willig,  Katrin. I.
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Willig, K. I. (2022). In vivo super-resolution of the brain - How to visualize the hidden nanoplasticity? iScience, 25(9): 104961. doi:10.1016/j.isci.2022.104961.

Cite as: https://hdl.handle.net/21.11116/0000-000C-0E90-2
Super-resolution fluorescence microscopy has entered most biological laboratories worldwide and its benefit is undisputable. Its application to brain imaging, for example in living mice, enables the study of sub-cellular structural plasticity and brain function directly in a living mammal. The demands of brain imaging on the different super-resolution microscopy techniques (STED, RESOLFT, SIM, ISM) and labeling strategies are discussed here as well as the challenges of the required cranial window preparation. Applications of super-resolution in the anesthetized mouse brain enlighten the stability and plasticity of synaptic nanostructures. These studies show the potential of in vivo super-resolution imaging and justify its application more widely in vivo to investigate the role of nanostructures in memory and learning.