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Journal Article

Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence

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Rosenberg,  UB
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Jäckle,  H
Department Biochemistry, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Schuh, R., Vestweber, D., Riede, I., Ringwald, M., Rosenberg, U., Jäckle, H., et al. (1986). Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence. Proceedings of the National Academy of Sciences of the United States of America, 83(5), 1364-1368. doi:10.1073/pnas.83.5.1364.


Cite as: https://hdl.handle.net/21.11116/0000-000C-05D9-A
Abstract
A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.