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Journal Article

Bacteriophage T7-DNA replication in vitro: an experimental system

MPS-Authors
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Strätling,  W
Knippers Group, Friedrich Miescher Laboratory, Max Planck Society;

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Ferdinand,  F-J
Knippers Group, Friedrich Miescher Laboratory, Max Planck Society;

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Krause,  E
Knippers Group, Friedrich Miescher Laboratory, Max Planck Society;

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Knippers,  R
Knippers Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Strätling, W., Ferdinand, F.-J., Krause, E., & Knippers, R. (1973). Bacteriophage T7-DNA replication in vitro: an experimental system. European Journal of Biochemistry, 38(1), 160-169. doi:10.1111/j.1432-1033.1973.tb03046.x.


Cite as: https://hdl.handle.net/21.11116/0000-000C-3FF7-8
Abstract
We describe an experimental system which consists of intact double-stranded T7 DNA and an unfractionated protein extract from bacteriophage-infected cells. Under proper conditions, DNA replication is initiate. Evidence will be presented that, at least the viral DNA polymerase and the function of the viral gene-4-protein are required for initiation. Circumstantial evidence suggests that the viral RNA polymerasee is also necessary for initiation.