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Cell envelope composition of Escherichia coli K12: a comparison of the cell poles and the lateral wall

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Goodell,  EW
Schwarz Group, Friedrich Miescher Laboratory, Max Planck Society;

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Schwarz,  U
Schwarz Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Goodell, E., Schwarz, U., & Teather, R. (1974). Cell envelope composition of Escherichia coli K12: a comparison of the cell poles and the lateral wall. European Journal of Biochemistry, 47(3), 567-572. doi:10.1111/j.1432-1033.1974.tb03727.x.


Cite as: https://hdl.handle.net/21.11116/0000-000C-6D5F-1
Abstract
Minicells and filaments of Escherichia coli strain P678–54 provide a convenient means of preparing cell-envelope fractions that are, respectively, greatly enriched or reduced in material derived from the cell poles. The inner (cytoplasmic) membrane, outer membrane, and sacculus were prepared both from minicells and from filaments purified from a single culture. One protein component and phosphatidylglycerol were found to be enriched in minicell inner membrane.