Process of cellular division in Escherichia coli: differention of growth zones 
in the Sacculus

Schwarz, U; Ryter, A; Rambach, A; Hellio, R; Hirota, Y

doi:10.1016/s0022-2836(75)80008-8

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Process of cellular division in Escherichia coli: differention of growth zones in the Sacculus

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Schwarz, U
Schwarz Group, Friedrich Miescher Laboratory, Max Planck Society;

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Citation

Schwarz, U., Ryter, A., Rambach, A., Hellio, R., & Hirota, Y. (1975). Process of cellular division in Escherichia coli: differention of growth zones in the Sacculus. Journal of Molecular Biology, 98(4), 749-759. doi:10.1016/s0022-2836(75)80008-8.

Cite as: https://hdl.handle.net/21.11116/0000-000C-6D6A-4

Abstract

The murein sacculus is part of the bacterial cell envelope; its mechanical stability enables the cell to retain its shape. The growth pattern of this structure was studied in Escherichia coli wild type and in a series of temperature-sensitive mutants with defects in cell division, DNA initiation and DNA elongation. The murein of cells growing in rich medium under permissive and non-permissive conditions was specifically pulse-labelled with [3H]diaminopimelic acid, and the distribution of label in isolated sacculi was analysed by high-resolution autoradiography. Two types of growth zones were found; one is assumed to be involved mainly in cell division, the other in cell elongation. Both can exist independently from each other as shown by the analysis of the autoradiographic grain patterns found in mutants.