A radiation hybrid map of the zebrafish genome

Geisler, R; Rauch, G-J; Baier, H; van Bebber, F; Broß, L; Dekens, MPS; Finger, K; Fricke, C; Gates, MA; Geiger, H; Geiger-Rudolph, S; Gilmour, D; Glaser, S; Gnügge, L; Habeck, H; Hingst, K; Holley, S; Keenan, J; Kirn, A; Knaut, H; Lashkari, D; Maderspacher, F; Martyn, U; Neuhauss, S; Neumann, C; Nicolson, T; Pelegri, F; Ray, R; Rick, JM; Roehl, H; Roeser, T; Schauerte, HE; Schier, AF; Schönberger, U; Schönthaler, H-B; Schulte-Merker, S; Seydler, C; Talbot, WS; Weiler, C; Nüsslein-Volhard, C; Haffter, P

doi:10.1038/12692

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A radiation hybrid map of the zebrafish genome

MPS-Authors

/persons/resource/persons273981

Geisler, R
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278351

Rauch, G-J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons39224

Baier, H
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278355

van Bebber, F
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285527

Broß, L
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278361

Dekens, MPS
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons86856

Finger, K
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285530

Fricke, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons275324

Geiger, H
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273978

Geiger-Rudolph, S
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285532

Gilmour, D
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278367

Glaser, S
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285534

Gnügge, L
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285536

Habeck, H
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285538

Hingst, K
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278369

Holley, S
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285540

Keenan, J
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278408

Kirn, A
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285542

Knaut, H
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273985

Maderspacher, F
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons281249

Martyn, U
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273990

Neuhauss, S
Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285545

Neumann, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278414

Nicolson, T
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285547

Pelegri, F
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278419

Ray, R
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278423

Rick, JM
Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285555

Roehl, H
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons15724

Roeser, T
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278427

Schauerte, HE
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons278432

Schönberger, U
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285550

Schönthaler, H-B
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274414

Schulte-Merker, S
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons285552

Seydler, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272426

Weiler, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271460

Nüsslein-Volhard, C
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons274411

Haffter, P
Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society;

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引用

Geisler, R., Rauch, G.-J., Baier, H., van Bebber, F., Broß, L., Dekens, M., Finger, K., Fricke, C., Gates, M., Geiger, H., Geiger-Rudolph, S., Gilmour, D., Glaser, S., Gnügge, L., Habeck, H., Hingst, K., Holley, S., Keenan, J., Kirn, A., Knaut, H., Lashkari, D., Maderspacher, F., Martyn, U., Neuhauss, S., Neumann, C., Nicolson, T., Pelegri, F., Ray, R., Rick, J., Roehl, H., Roeser, T., Schauerte, H., Schier, A., Schönberger, U., Schönthaler, H.-B., Schulte-Merker, S., Seydler, C., Talbot, W., Weiler, C., Nüsslein-Volhard, C., & Haffter, P. (1999). A radiation hybrid map of the zebrafish genome. Nature Genetics, 23(1), 86-89. doi:10.1038/12692.

引用: https://hdl.handle.net/21.11116/0000-000C-6FE0-B

要旨

Recent large-scale mutagenesis screens have made the zebrafish the first vertebrate organism to allow a forward genetic approach to the discovery of developmental control genes(1-3). Mutations can be cloned positionally, or placed on a simple sequence length polymorphism (SSLP) map(4-6) to match them with mapped candidate genes and expressed sequence tags(7,8) (ESTs). To facilitate the mapping of candidate genes and to increase the density of markers available for positional cloning, we have created a radiation hybrid (RH) map of the zebrafish genome. This technique is based on somatic cell hybrid lines produced by fusion of lethally irradiated cells of the species of interest with a rodent cell line. Random fragments of the donor chromosomes are integrated into recipient chromosomes or retained as separate minichromosomesg (9,10) The radiation-induced breakpoints can be used for mapping in a manner analogous to genetic mapping, but at higher resolution and without a need for polymorphism. Genome-wide maps exist for the human, based on three RH panels of different resolutions(11-13), as well as for the dog(14) rat(15) and mouse(16, 17). For our map of the zebrafish genome, we used an existing RH panel (18, 19) and 1,451 sequence tagged site (STS) markers, including SSLPs, cloned candidate genes and ESTs. Of these, 1,275 (87.9%) have significant linkage to at least one other marker. The fraction of ESTs with significant linkage, which can be used as an estimate of map coverage, is 87.9%. We found the average marker retention frequency to be 18.4%. One cR(3000) is equivalent to 61 kb, resulting in a potential resolution of approximately 350 kb.