Cloning and overexpressing of phycoerythrocyanin operon F-gene of Mastigocladus 
laminosus in Escherichia coli

Zheng, M; Da, L; Deng, MG; Qing, YM; Zhou, M; Zhao, KH

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Cloning and overexpressing of phycoerythrocyanin operon F-gene of Mastigocladus laminosus in Escherichia coli

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http://ssswxb.ihb.ac.cn/article/app/id/4b49730d-d505-454c-aa89-b08b314ef1aa?pageType=en
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Citation

Zheng, M., Da, L., Deng, M., Qing, Y., Zhou, M., & Zhao, K. (2000). Cloning and overexpressing of phycoerythrocyanin operon F-gene of Mastigocladus laminosus in Escherichia coli. Acta Hydrobiologica Sinica, 26(2), 168-174.

Cite as: https://hdl.handle.net/21.11116/0000-000C-72EE-8

Abstract

By PCR method, apophycoeryt hrocyanin operon F-gene (p ecF) of Mastigocladus laninosus was amplified from its genom icDNA, and then cloned in pBlue-script. The pecF gene was subcloned into the expression vector pGEM D, and then transformed into E. coli BL 21 (DE3) . After induction, a new protein of molecular weight 22. 5k Da existing in inclusion body was overexpressed. The expression product was confirmed to possess phycoerythrocyanin lyase activity.