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Journal Article

Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing.

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Winkler,  Sylke
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Dahn, H. A., Mountcastle, J., Balacco, J., Winkler, S., Bista, I., Schmitt, A. D., et al. (2022). Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing. GigaScience, 11: giac068. doi:10.1093/gigascience/giac068.


Cite as: https://hdl.handle.net/21.11116/0000-000C-7445-4
Abstract
Studies in vertebrate genomics require sampling from a broad range of tissue types, taxa, and localities. Recent advancements in long-read and long-range genome sequencing have made it possible to produce high-quality chromosome-level genome assemblies for almost any organism. However, adequate tissue preservation for the requisite ultra-high molecular weight DNA (uHMW DNA) remains a major challenge. Here we present a comparative study of preservation methods for field and laboratory tissue sampling, across vertebrate classes and different tissue types.