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Journal Article

Organoid Imaging: Seeing Development and Function.

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Keshara,  Rashmiparvathi
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Kim,  Yung Hae
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Grapin-Botton,  Anne
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Keshara, R., Kim, Y. H., & Grapin-Botton, A. (2022). Organoid Imaging: Seeing Development and Function. Annual review of cell and developmental biology, 38, 447-466. doi:10.1146/annurev-cellbio-120320-035146.


Cite as: https://hdl.handle.net/21.11116/0000-000C-7459-E
Abstract
Organoids are miniaturized and simplified versions of an organ produced in vitro from stem or progenitor cells. They are used as a model system consisting of multiple cell types forming an architecture relevant to the organ and carrying out the function of the organ. They are a useful tool to study development, homeostasis, regeneration, and disease. The imaging of organoids has become a pivotal method to visualize and understand their self-organization, symmetry breaking, growth, differentiation, and function. In this review, we discuss imaging methods, how to analyze these images, and challenges in organoid research.