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Book Chapter

Tight-Seal Whole-Cell Recording


Marty,  A.
Research Group of Cellular Neurobiology, MPI for biophysical chemistry, Max Planck Society;


Neher,  Erwin       
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Marty, A., & Neher, E. (1995). Tight-Seal Whole-Cell Recording. In B. Sakmann, & E. Neher (Eds.), Single-Channel Recording (pp. 31-52). New York: Springer.

Cite as: https://hdl.handle.net/21.11116/0000-000C-756C-8
The tight-seal whole-cell recording method, often abbreviated as “whole-cell recording” (WCR), allows one to record from cells and modify their internal environment by using a patch-clamp pipette. This has become the most commonly used configuration of the patch-clamp technique. In the present chapter, we first describe the basic experimental procedures used to obtain whole-cell recordings. We then discuss the pipette—cell interactions during whole-cell recording, first from an electrical point of view and then from a chemical point of view. We finally compare the tight-seal whole-cell recording with other methods for studying electrical properties of cells.