Transformation of Medicago truncatula via infiltration of seedlings or 
flowering plants with Agrobacterium

Trieu, AT; Burleigh, SH; Kardailsky, IV; Maldonado-Mendoza, IE; Versaw, WK; Blaylock, LA; Shin, H; Chiou, TJ; Katagi, H; Dewbre, GR; Weigel, D; Harrison, MJ

doi:10.1046/j.1365-313x.2000.00757.x

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Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

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Trieu, A., Burleigh, S., Kardailsky, I., Maldonado-Mendoza, I., Versaw, W., Blaylock, L., et al. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. The Plant Journal, 22(6), 531-541. doi:10.1046/j.1365-313x.2000.00757.x.

Cite as: https://hdl.handle.net/21.11116/0000-000C-7EFC-C

Abstract

Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.